Responses of Potato (Solanum tuberosum L.) Breeding Lines to Osmotic Stress Induced in In Vitro Shoot Culture

In vitro experiments were conducted to study the responses of potato (Solanum tuberosum L.) genotypes osmotic stress. shoot cultures 27 breeding lines and their drought-tolerant parents (referent lines: C103 C107) tested under stress induced by addition PEG 6000 (Mw = 6000; 5.0, 7.5, 10.0%, w/v), D-mannitol (0.1, 0.2, 0.3 M) 600 600; 2.5, 7.5%, w/v) Murashige-Skoog medium. Stress index (SI) was calculated from length (SL) root (RL), numbers (RN) rate surviving shoots (SR) (SISL;RL;RN;SR ParameterSL;RL;RN;SR treated shoots/ParameterSL;RL;RN;SR control × 100) compare genotypes. average each line concentration, agents resulted in SI values 40.1, 60.8, 82.6 76.0 for SISL, SIRL, SIRN SISR, respectively. general, all SIRL,RN C107 significantly higher than those lines. Nine found be promising based on final ranking. According results, 7.5% 10% or 0.2 M treatments proved suitable selection stress-tolerant